From: A round robin approach to the analysis of bisphenol a (BPA) in human blood samples
 | Laboratory # 2 | Laboratory # 3 | Laboratory # 4 |
---|---|---|---|
Method source | US CDC [46] | US FDA/NCTR [76] | Designed with high enzyme concentration |
Enzyme source | Helix pomatia | Helix pomatia Type H-3 | Helix pomatia Type H-1 |
Enzyme info | Sigma-Aldrich | Sigma-Aldrich | Sigma Aldrich, G 0751 |
Number of units used | 291.4 U | 2 U | 1000 U |
Volume of sample | 0.5Â ml | 0.25Â ml | 1Â ml |
pH of reaction | 5 | 5.5 | 5 |
Length of reaction | 12Â h | 2Â h | Overnight (~18Â h) |
Temperature of reaction | 37°C | 37°C | 37°C |