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Table 4 Characteristics of enzymatic treatments used in indirect measures

From: A round robin approach to the analysis of bisphenol a (BPA) in human blood samples

 

Laboratory # 2

Laboratory # 3

Laboratory # 4

Method source

US CDC [46]

US FDA/NCTR [76]

Designed with high enzyme concentration

Enzyme source

Helix pomatia

Helix pomatia Type H-3

Helix pomatia Type H-1

Enzyme info

Sigma-Aldrich

Sigma-Aldrich

Sigma Aldrich, G 0751

Number of units used

291.4 U

2 U

1000 U

Volume of sample

0.5 ml

0.25 ml

1 ml

pH of reaction

5

5.5

5

Length of reaction

12 h

2 h

Overnight (~18 h)

Temperature of reaction

37°C

37°C

37°C