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Figure 2 | Environmental Health

Figure 2

From: Disruption of human plasma cell differentiation by an environmental polycyclic aromatic hydrocarbon: a mechanistic immunotoxicological study

Figure 2

B [ a ]P suppresses plasma cell production. Purified CD20+ B cells from 4 donors were individually cultured in two steps as in Figure 1 to generate plasma cells. Cultures were treated with vehicle or 10-6 M B [a]P during the first (B [a]P/VEH), the second (VEH/B [a]P), or both (B [a]P/B [a]P) culture periods. A) Dot plots of cells obtained from a representative donor stained with CD20- and CD38-specific or isotype control antibodies are shown. Quadrants were set using isotype control antibodies. The percentages of CD20lo/CD38hi plasma cells are indicated in the upper left quadrants. B) The percentages of CD20lo/CD38hi plasma cells obtained after the second culture period when cells were exposed to vehicle or B [a]P during the first, second, or both cultures are presented as means + SE. C) The total number of CD20lo/CD38hi plasma cells obtained after both cultures (day 8) are presented as means + SE. D) The total number of cells obtained after both cultures are presented as mean + SE. An asterisk (*) indicates a significant decrease relative to vehicle-treated cells (p < 0.05; Dunnett's).

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