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Figure 3 | Environmental Health

Figure 3

From: Disruption of human plasma cell differentiation by an environmental polycyclic aromatic hydrocarbon: a mechanistic immunotoxicological study

Figure 3

γ-Irradiation inhibits cell growth but has no effect on B cell differentiation into plasma cells. Purified CD20+ B cells were activated for 4 days as described in Figure 1. Activated B cells were then γ-irradiated (700 Rads) and cultured without CD40L-transfected cells but with IL-2, IL-6, IL-10, IL-12 and IFN-α. A) [3H]-thymidine was added to cultures of irradiated and control cells at 21 hours and [3H]-thymidine incorporation assayed 18 hours later. Data from 3 donors are presented as mean cpm ± SE. An asterisk indicates a significant decrease in [3H]-thymidine incorporation as compared with corresponding non-irradiated controls (p < 0.05; paired t-test). B) Cells cultured as in "A" above were phenotyped for CD20 and CD38 expression by flow cytometry 4 days after irradiation and culture under plasma cell differentiation conditions. Dot plots obtained with cells from a representative donor are shown. The percentage of CD20lo/CD38hi plasma cells is indicated in the upper left quadrant.

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