Figure 3From: Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells Changes in calcium responses elicited by physiologic estrogens (PE) and xenoestrogens (XE), alone and in combination. The level of Ca [compared to the pretreatment baseline (B) measurement for each assessed cell] was measured by Fura-2 imaging in cells treated with 10 pM E2, E1, or E3 or different 10 pM alkylphenols (EP, PP, OP, NP, or BPA), or physiologic estrogen and xenoestrogen combinations. (A-C) % of cells responding to estrogens; (D-F) changes in accumulated intracellular calcium levels; (G-I) changes of calcium oscillation frequency. Note - some baseline measurements (D-F) were too low to show up as bars on the graphs. * = p < 0.05 compared to control cells. # = p < 0.05 compared to E2, E1, or E3 alone.Back to article page